Journal: eLife

Article Title: TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development

doi: 10.7554/eLife.80405

Figure Lengend Snippet:

Article Snippet: RNeasy Plus Micro Kit (Qiagen, 74034) was used to isolate RNA.

Techniques: Sequencing, RNAscope, Plasmid Preparation, Recombinant, Multiplex Assay, cDNA Synthesis, Software

Journal: Cell reports

Article Title: Pain and itch coding mechanisms of polymodal sensory neurons

doi: 10.1016/j.celrep.2023.113316

Figure Lengend Snippet:

Article Snippet: Media was then removed, and the pellet was gently washed once with HBSS. mRNA from isolated cells were purified, and cDNA library were compiled using First-Strand enzyme Kit (NEB, E6300S) and PCR was performed using the Taq DNA Polymerase kit (NEB, E5000S) using the manufacturer’s recommended protocols.

Techniques: Recombinant, Blocking Assay, Glutamate Assay, Enzyme-linked Immunosorbent Assay, RNAscope, Multiplex Assay, Software

Journal: Cell reports

Article Title: Circulating monocytes associated with anti-PD-1 resistance in human biliary cancer induce T cell paralysis

doi: 10.1016/j.celrep.2022.111384

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The RNeasy Mini Kit (Qiagen) was used to extract RNA from minimum 2.5 × 10 5 cells per PBMC sample. cDNA was prepared using methods previously described, with the Smart-seq2 protocol , and libraries were prepared using Nextera XT DNA Sample Preparation Kit.

Techniques: Control, Recombinant, Staining, Blocking Assay, Sample Prep, RNAscope, Multiplex Assay, Biomarker Discovery, Software, Cytometry, Microscopy

Journal: Cell reports

Article Title: A Leptin-Mediated Neural Mechanism Linking Breathing to Metabolism

doi: 10.1016/j.celrep.2020.108358

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Parlow SG substrate kit peroxidase Vector laboratories Cat.# SK-4700; RRID:AB_2314425 Vectamount Vector laboratories Cat.# H-5000; RRID:AB_2336786 Critical Commercial Assays Mouse Leptin ELISA kit Crystal Chem, USA Cat.# 90030 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio, USA Cat.# 634894 Nextera XT DNA Library Preparation Kit Illumina, USA FC-131–1024 RNAscope 2.5 High Definition BROWN Assay kit Advanced Cell Diagnostics, USA Cat.# 322371 RNAscope 2.5 Duplex Assay kit Advanced Cell Diagnostics, USA Cat.# 322436 Deposited Data LepRb NTS type1 and type2 single-cell RNA- sequencing data Gene Expression Omnibus GSE158582 Experimental Models: Organisms/Strains B6.129(Cg)-Lepr tm2(cre)Rck /J Jackson Laboratories, USA RRID:IMSR_JAX:008320 B6;129S-Gt(ROSA)26Sor tm32(CAG-COP4 * H134R/EYFP)Hze / J (Ai32) Jackson Laboratories, USA RRID:IMSR_JAX:024109 B6;129S-Gt(ROSA)26Sor tm39(CAG-hop/EYFP)Hze /J (Ai39) Jackson Laboratories, USA RRID:IMSR_JAX:014539 C57;B6 loxP-Tdt::loxP-NALCN Deijen Ren, UPenn Lu et al., 2007 Oligonucleotides Mm-Gcg-O1 Advanced Cell Diagnostics, USA Ref. 482311 Mm-CCK Advanced Cell Diagnostics, USA Ref. 402271 Mm-POMC Advanced Cell Diagnostics, USA Ref. 314081 Mm-Gal Advanced Cell Diagnostics, USA Ref. 400961 Mm-Gal-C2 Advanced Cell Diagnostics, USA Ref. 400961-C2 Mm-NALCN Advanced Cell Diagnostics, USA Ref. 415161 Mm-Lepr Advanced Cell Diagnostics, USA Ref. 402731 Mm-Lepr-tv1-C2 Advanced Cell Diagnostics, USA Ref. 471171-C2 Software and Algorithms Clampfit10.2 Axon Instruments https://www.moleculardevices.com MATLAB MathWorks https://www.mathworks.com Prism5 GraphPad https://www.graphpad.com Other Temperature transponder BMDS, USA IPTT-300 Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Recombinant, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Sequencing, DNA Library Preparation, RNAscope, RNA Sequencing, Gene Expression, Software